Detection of a single DNA base-pair mismatch using an anthracene-tagged fluorescent probe.
نویسندگان
چکیده
A novel anthracene-tagged oligonucleotide can discriminate between a fully-matched DNA target sequence and one with a single mismatching base-pair through a remarkable difference in fluorescence emission intensity upon duplex formation.
منابع مشابه
Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe.
A fluorescent DNA probe containing an anthracene group attached via an anucleosidic linker can identify all four DNA bases at a single site as well as the epigenetic modification C/5-MeC via a hybridisation sensing assay.
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عنوان ژورنال:
- Chemical communications
دوره 48 شماره
صفحات -
تاریخ انتشار 2006